Tumor Model in Exercised or Sedentary Rats
In this study, we are using Fischer 344 rats injected with Mat B III breast cancer cells purchased from the American Type Culture Collection (ATCC). The study consists of exercised and sedentary rats, within each group there will be tumor bearing and non-tumor bearing animals. The rats will then be observed for 14-21 days post injection or until the tumor measures three centimeters in any directions. Following the post injection period, the bone marrow and blood of the rats will be harvested and treated with His48 and CD11bc antibodies to determine the amount of MDSCs present in the cells of the rats.
Exercise
The exercise for the experimental rats will consist of voluntary wheel running, whereas the control group will be sedentary. An electronic sensor is attached to each wheel to allow for daily monitoring of the rats’ exercise. The animals will be given wheels four weeks prior to inoculation in order to condition them to daily exercise. This exercise will continue for approximately seven weeks until the rats are euthanized.
In this study, we are using Fischer 344 rats injected with Mat B III breast cancer cells purchased from the American Type Culture Collection (ATCC). The study consists of exercised and sedentary rats, within each group there will be tumor bearing and non-tumor bearing animals. The rats will then be observed for 14-21 days post injection or until the tumor measures three centimeters in any directions. Following the post injection period, the bone marrow and blood of the rats will be harvested and treated with His48 and CD11bc antibodies to determine the amount of MDSCs present in the cells of the rats.
Exercise
The exercise for the experimental rats will consist of voluntary wheel running, whereas the control group will be sedentary. An electronic sensor is attached to each wheel to allow for daily monitoring of the rats’ exercise. The animals will be given wheels four weeks prior to inoculation in order to condition them to daily exercise. This exercise will continue for approximately seven weeks until the rats are euthanized.